Description
It is widely used in protocols for the isolation of proteins
1 g/cm³ at 25 °C
bases and alcoholic solutions
Addition of 1 volume ROTI®Phenol/Chloroform/Isoamyl alcohol and thorough mixing
TAE Buffer Rotiphorese Size:5L It is widely used inROTIPHORESE10x TAE Buffer ROTIPHORESE 10x conc. ROTIPHORESE10x TAE Buffer contains 0,4 M Tris, 0,2 M acetic acid and 10 mM EDTA in distilled, deionised water; pH 8,3. The TAE buffer is optimally suited as a gel and running buffer system. TAE buffer has a lower buffer capacity than TBE and can be used with higher voltages, longer gels and larger DNA fragments. The gels heat up during running to a lesser extent than when using the running buffer TBE.
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