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acetylcholine ring | silver US Size:6 partly because the survival storyacetylcholine ring sterling silver The first neurotransmitter ever discovered. Otto Loewi proved its existence in 1921 with an elegant experiment: collecting fluid from a stimulated frog heart and transferring it to a resting heart, which then slowed. That experiment earned the 1936 Nobel Prize and launched modern neuroscience. The Science Behind Acetylcholine Acetylcholine (ACh) is synthesized from choline and acetyl CoA by choline acetyltransferase